Richard Fahlman

Research:

Post-transcriptional regulation of the Cellular Proteome:

Much of our research focuses on cellular processes that regulate the cellular proteome. These include gene silencing by the small miRNAs and protein degradation by the N-End rule pathway.

Proteomic Applications:

As our investigations on the biological process of both mi RNAs and protein degradation have been revolving more around mass spectrometry and proteomic applications, our research interests have also begun to include broader proteome wide applications. These include the development of new techniques to applying these techniques to new biological systems.

Selected Publications:

The molecular basis for the post-translational addition of amino acids by L/F transferase in the N-End rule pathway.
Fung AWS. Fahlman RP.
Current Prot. Peptide Science (2015) 16:163-180.

The Determination of tRNALeu Recognition Nucleotides for Escherichia coli L/F Transferase.
Fung AWS, Leung CCY, Fahlman RP.
RNA (2014) 20: 1210-1222

The anti-apoptotic form of Tyrosine Kinase Lyn that is Generated by Proteolysis is Degraded by the N-End Rule Pathway.
Eldeeb M, Fahlman RP.
Oncotarget (2014) 5: 2714-2735.

MicroRNA miR-92a-1 Biogenesis and mRNA Targeting are Modulated by a Tertiary Contact Within the miR-17~92 Cluster.
Chaulk SG, Xu Z, Glover JN and Fahlman RP.
Nucleic Acids Res. (2014) 42: 5234-5244.

The Hippo Pathway Effectors TAZ/YAP Regulate Dicer Expression and miRNA Biogenesis Through Let-7.
Chaulk SG, Lattanzi VJ, Hiemer SE, Fahlman RP, Varelas X.
J. Biol. Chem. (2014) 289: 1886-1891.

Absolute Proteomic Quantification of the Activity State of Proteases and Proteolytic Cleavages Using Proteolytic Signature Peptides and Isobaric Tags.
Fahlman RP, Chen W, Overall CM.
J. Proteomics (2014) 100: 79-91.

The C-terminal proteolytic fragment of the breast cancer susceptibility type I protein (BRCA1) is degraded by the N-end rule pathway.
Xu Z, Payoe R, Fahlman RP.
J. Biol. Chem. (2012) 287(10):7495-7502.

Role of pri-miRNA Tertiary Structure in miR-17~92 miRNA Biogenesis.
Chaulk SG, Thede GL, Kent OA, Xu Z, Gesner EM, Veldhoen RA, Khanna SK, Goping IS, MacMillan AM, Mendell JT, Young HS, Fahlman RP, Glover JNM.
RNA Biology (2011) 8(6):1105 - 1114.