Olivier Julien

Research:

The central goal of our research is to characterize how proteases regulate important biological processes such as apoptosis and cell differentiation. For example, the dysregulation of caspases underlies several human diseases that include cancer and inflammatory disorders, and a better understanding of these enzymes is of great importance in order to design new therapies. Using functional proteomics approaches, our lab focus on characterizing how protease substrates play critical roles in such vital processes, to advances our knowledge about the role of these proteases in human diseases. Harnessing recent progress in cellular engineering, we take advantage of tools such as CRISPRi technology and antibody engineering to further support our proteomics findings.

Selected Publications:

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts
Hartley B, Bassiouni W, Roczkowsky A, Fahlman R, Schulz R, Julien O 
J. Proteome Research. (2024) 23:844-856

Application of N-terminal labeling methods provide novel insights into endoproteolysis of the prion protein in vivo
Gomez-Cardona E, Eskandari-Sedighi G, Fahlman R, Westaway D, Julien O 
ACS Chemical Neuroscience. (2024) 15:134-146

Identification of human host substrates of the SARS-CoV-2 Mpro and PLpro using subtiligase N-terminomics
Luo SH, Moussa E, Ishida R, Orozco-Lopez J, Wang H, Wilson J, Kumar A, Hobman T, Julien O 
ACS Infectious Diseases (2023) 9:749-61  

Deorphanizing caspase-3 and caspase-9 substrates in and out of apoptosis with deep substrate profiling
Araya LE, Soni IV, Hardy J, Julien O 
ACS Chem. Biol. (2021) 16:2280-2296  

Protease substrate identification using N-terminomics
Luo SH, Araya LE, Julien O 
ACS Chem. Biol. (2019) 14:2361-2371  

The Unique Cofactor Region of Zika Virus NS2B-NS3 Protease Facilitates Cleavage of Key Host Proteins.
Hill ME, Kumar A, Wells JA, Hobman TC, Julien O, Hardy JA.
ACS Chem Biol. 2018 Aug 14. doi: 10.1021/acschembio.8b00508. [Epub ahead of print]

Caspase substrates in cell death and remodeling.
Julien O, Wells JA.
Cell Death Differ (2017) 24:1380-1389