Fee Schedule
| Service | Description | Fee |
| Mouse Sperm Cryopreservation | User will supply one proven breeder male (ideally <6 months old) to the facility. Sperm will be harvested and approximately nine (9) aliquots of sample frozen. A basic motility check will be performed for quality assessment. | $300 |
| Mouse Embryo Cryopreservation | User will supply 10 females (3-8 weeks of age) and 10 males (over 8 weeks of age). Approximately 200 embryos will be cryopreserved however only a portion of these will be viable after thawing. | $500 |
| Mouse Sperm Storage | Annual fee to store samples under liquid nitrogen. Aliquots of each sample will be divided among two tanks. | $100 |
| in vitro Fertilization | Unfertilized oocytes collected from five (5) FVB* females and mixed with sperm. Fertilized oocytes transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping. | $2,100 |
| Pronuclear Injection (Transgenic Mouse Production) |
Three (3) rounds of injection, collecting from eight (8) mice each round. Approximately 300 embryos collected. Surviving embryos transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping. | $4,800 |
| CRISPR/Cas9 | Three (3) rounds of injection and/or electroporation, collecting from eight (8) mice each round. Approximately 300 embryos collected. Surviving embryos transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping. NOTE: All CRISPR/Cas9 reagents to be provided by user. | $4,200 |
| Rederivation | Unfertilized oocytes from five commercially-available females are collected and mixed with sperm from the male of the "contaminated" stain. Embryos are thoroughly washed and transferred into pathogen-free recipients. Pups will remain with the recipient female until weaning. Following weaning, Mouse Assessment Plus serology of the dams and PCR Infectious Agent (PRIA) testing of both the dams and pups will be completed by Charles River Laboratories. |
$3,000 *Additional fees may occur if extended housing or additional testing required. |