Molecular Biology Facility (MBSU)

Documents & Manuals

This page contains documentation that facility users may find useful when it comes to understanding how the equipment found in the Microarray and Proteomics Facility (MAF) work.

Additionally, many of these documents contain useful protocols that can serve as starting points or standalone recipes for carrying out protocols prior to using MAF equipment.

Equipment	Manuals
Real Time PCR	Real Time PCR vs. Traditional PCR Essentials of Real Time PCR ABI Real Time PCR Chemistry Guide Relative Quantitation Getting Started Guide (Advanced Topic) Relative Quantitation Experimental Guide (Advanced Topic)
2-Dimensional Gel Electrophoresis Manuals	Amersham Guide to 2D Gel Electrophoresis Fluroescence Imaging Principles Ettan DIGE System Users Manual DeCyder Version 5.0 Users Manual ImageQuant 5.0 Tutorial ImageQuant 5.0 Users Manual ImageQuant 5.0 Utilities FLA-5000 Science Lab for Windows FLA-5000 Science Lab for Mac FLA-5000 Multigauge Version 2.0 for Windows Fluroescence Imaging Principles
Protein Purification and FPLC	General Protein Purification Principles Principles of Affinity Chromatography Antibody Purification Principles Gel Filtration Chromatography Principles GST Fusion Protein System Hydrophobic Interaction Chromatography Principles Ion Exchange Chromatography Principles Reverse Phase Chromatography Principles
Agilent 2100 Bioanalyzer	Bioanalyzer 2100 Expert Software Manual Nano Scale RNA Chip Manual Nano Scale RNA Chip Quick Reference Guide Pico Scale RNA Chip Manual Pico Scale RNA Chip Quick Reference Guide
Microarray Products	Amersham Microarray Handbook Corning Epoxide Slide Manual Corning GAPSII Slide Manual Corning UltraGAPS Manual
Microarray Scanners	Array Worx Manual
Nanodrop Spectrophotometer	Nanodrop Spec 1000 Users Manual

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